Details of Host Protein-DME Interaction (HOSPPI)

General Information of Drug-Metabolizing Enzyme (DME ID: DME0023)
DME Name	Cytochrome P450 1B1 (CYP1B1), Homo sapiens	DME Info
UniProt ID	CP1B1_HUMAN
EC Number	EC: 1.14.14.1 (Click to Show/Hide the Complete EC Tree) Oxidoreductase Oxygen paired donor oxidoreductase Flavin/flavoprotein donor oxidoreductase EC: 1.14.14.1
Lineage	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Interactome

Disease Specific Interactions between Host Protein and DME (HOSPPI)
ICD Disease Classification Healthy
ICD-11: Healthy		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Oligomerization
Cytochrome b5 (CYB5A)		Health		Heterodimer
Uniprot ID	CYB5_HUMAN
Interaction Name	CYB5A-CYP1B1 heterodimerization				[1]
Studied Cell Lines	Escherichia coli cell line
Description	Cytochrome b5 (CYB5A) is reported to heterodimerize with the CYP1B1 protein, which has no effect on the enzyme activity of Cytochrome P450 1B1. As a result, the interaction between CYB5A and CYP1B1 can have no effect on the drug-metabolizing process of Cytochrome P450 1B1.
ICD Disease Classification 02 Neoplasms
ICD-11: 2B30 Lymphoma		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Lymphoma		Significant hypermethylation
Interaction Name	DNMT-CYP1B1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 6.85E-35; delta-beta: 5.95E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the CYP1B1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between DNMT and CYP1B1 can significantly affect the drug-metabolizing process of Cytochrome P450 1B1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2B5F Uterine carcinosarcoma		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Uterine carcinosarcoma		Significant hypermethylation
Interaction Name	DNMT-CYP1B1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 8.99E-06; delta-beta: 3.32E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the CYP1B1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between DNMT and CYP1B1 can significantly affect the drug-metabolizing process of Cytochrome P450 1B1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2B90 Colorectal cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Transcription-factor regulation
Transcription factor Sp1 (SP1)		Colorectal cancer		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-CYP1B1 interaction				[2]
Studied Cell Lines	LS-180 cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between SP1 and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
ICD-11: 2C12 Liver cancer		Click to Show/Hide the Full List of HOSPPI: 2 HOSPPI
Transcription-factor regulation
Transcription factor Sp1 (SP1)		Liver cancer		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-CYP1B1 interaction				[2]
Studied Cell Lines	HepG2 cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between SP1 and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
Non-coding RNA regulation
hsa-miR-27b-3p		Liver cancer		Suppression
miRBase ID	MIMAT0000419
Interaction Name	hsa-miR-27b-3p--CYP1B1 regulation				[3]
Studied Cell Lines	HepG2 cell line
Description	hsa-miR-27b-3p is reported to suppress CYP1B1 mRNA translation by binding to the 3' untranslated region (3'UTR) of CYP1B1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1.
ICD-11: 2C25 Lung cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Non-coding RNA regulation
hsa-miR-187-5p		Lung cancer		Suppression
miRBase ID	MIMAT0004561
Interaction Name	hsa-miR-187-5p--CYP1B1 regulation				[4]
Studied Cell Lines	A549 and SPC-A-1 cell lines
Description	hsa-miR-187-5p is reported to suppress CYP1B1 mRNA translation by binding to the 3' untranslated region (3'UTR) of CYP1B1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1.
ICD-11: 2C30 Melanoma		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Melanoma		Significant hypermethylation
Interaction Name	DNMT-CYP1B1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 1.51E-04; delta-beta: 4.81E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the CYP1B1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between DNMT and CYP1B1 can significantly affect the drug-metabolizing process of Cytochrome P450 1B1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2C60 Breast cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Transcription-factor regulation
Transcription factor Sp1 (SP1)		Breast cancer		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-CYP1B1 interaction				[2]
Studied Cell Lines	MCF-7 cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between SP1 and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
ICD-11: 2C73 Ovarian cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Transcription-factor regulation
Transcription factor Sp1 (SP1)		Ovarian cancer		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-CYP1B1 interaction				[2]
Studied Cell Lines	OMC-3 cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between SP1 and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
ICD-11: 2C77 Cervical cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Histone modification
Histone deacetylases (HDACs)		Cervical cancer		Repression
Uniprot ID	HDAC1_HUMAN
Interaction Name	HDACs-CYP1B1 interaction				[5]
Studied Cell Lines	HeLa cell line
Description	Histone deacetylases (HDACs) are reported to deacetylate the CYP1B1 gene and thereby repress the transcriptional activity of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between HDACs and CYP1B1 can inhibit the drug-metabolizing process of Cytochrome P450 1B1.
ICD-11: 2C82 Prostate cancer		Click to Show/Hide the Full List of HOSPPI: 2 HOSPPI
Transcription-factor regulation
Aryl hydrocarbon receptor (AHR)		Prostate cancer		Activation
Uniprot ID	AHR_HUMAN
Interaction Name	AHR-CYP1B1 interaction				[6], [7]
Studied Cell Lines	DU145, DUPro, LNCaP, PC-3 and ND-1 cell lines
Ensembl ID	ENSG00000106546
Description	Aryl hydrocarbon receptor (AHR) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between AHR and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
Hypoxia-inducible factor 1-beta (ARNT)		Prostate cancer		Activation
Uniprot ID	ARNT_HUMAN
Interaction Name	ARNT-CYP1B1 interaction				[6], [7]
Studied Cell Lines	DU145, DUPro, LNCaP, PC-3 and ND-1 cell lines
Ensembl ID	ENSG00000143437
Description	Hypoxia-inducible factor 1-beta (ARNT) is reported to activate the transcription of CYP1B1 gene, which leads to an increased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between ARNT and CYP1B1 can activate the drug-metabolizing process of Cytochrome P450 1B1.
ICD-11: 2C90 Renal cell carcinoma		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Non-coding RNA regulation
hsa-miR-200c-5p		Renal cell carcinoma		Suppression
miRBase ID	MIMAT0004657
Interaction Name	hsa-miR-200c-5p--CYP1B1 regulation				[8]
Studied Cell Lines	Renal cell carcinoma cell line
Description	hsa-miR-200c-5p is reported to suppress CYP1B1 mRNA translation by binding to the 3' untranslated region (3'UTR) of CYP1B1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1.
ICD-11: 2E06 Prostate cancer metastasis		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Prostate cancer metastasis		Significant hypermethylation
Interaction Name	DNMT-CYP1B1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 3.27E-03; delta-beta: 4.77E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the CYP1B1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Cytochrome P450 1B1. As a result, the interaction between DNMT and CYP1B1 can significantly affect the drug-metabolizing process of Cytochrome P450 1B1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual

References
1	Thermodynamics of interactions between mammalian cytochromes P450 and b5. Arch Biochem Biophys. 2017 Apr 1;619:10-15.
2	Critical enhancer region to which AhR/ARNT and Sp1 bind in the human CYP1B1 gene. J Biochem. 2003 May;133(5):583-92.
3	Fumonisin B?modulates expression of human cytochrome P450 1b1 in human hepatoma (Hepg2) cells by repressing Mir-27b. Toxicol Lett. 2014 May 16;227(1):50-5.
4	MicroRNA-187-5p suppresses cancer cell progression in non-small cell lung cancer (NSCLC) through down-regulation of CYP1B1. Biochem Biophys Res Commun. 2016 Sep 16;478(2):649-55.
5	Effects of histone deacetylation and DNA methylation on the constitutive and TCDD-inducible expressions of the human CYP1 family in MCF-7 and HeLa cells. Toxicol Lett. 2003 Sep 30;144(2):247-56.
6	Cytochrome P450 1B1 is overexpressed and regulated by hypomethylation in prostate cancer. Clin Cancer Res. 2005 Aug 15;11(16):5793-801.
7	CYP1B1, but not CYP1A1, is downregulated by promoter methylation in colorectal cancers. Int J Oncol. 2009 Apr;34(4):1085-91.
8	Loss of miR-200c up-regulates CYP1B1 and confers docetaxel resistance in renal cell carcinoma. Oncotarget. 2015 Apr 10;6(10):7774-87.

If you find any error in data or bug in web service, please kindly report it to Dr. Yin and Dr. Li.