Details of Host Protein-DME Interaction (HOSPPI)

General Information of Drug-Metabolizing Enzyme (DME ID: DME0111)
DME Name	Ecto-5'-nucleotidase (NT5E), Homo sapiens	DME Info
UniProt ID	5NTD_HUMAN
EC Number	EC: 3.1.3.5 (Click to Show/Hide the Complete EC Tree) Hydrolases Ester bond hydrolase Phosphoric monoester hydrolase EC: 3.1.3.5
Lineage	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Interactome

Disease Specific Interactions between Host Protein and DME (HOSPPI)
ICD Disease Classification 02 Neoplasms
ICD-11: 2B30 Lymphoma		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Lymphoma		Significant hypermethylation
Interaction Name	DNMT-NT5E interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 1.57E-23; delta-beta: 4.91E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the NT5E gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Ecto-5'-nucleotidase. As a result, the interaction between DNMT and NT5E can significantly affect the drug-metabolizing process of Ecto-5'-nucleotidase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2B90 Colorectal cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Transcription-factor regulation
ARNT-interacting protein (HIF1A)		Colorectal cancer		Activation
Uniprot ID	HIF1A_HUMAN
Interaction Name	HIF1A-NT5E interaction				[1]
Studied Cell Lines	T84 cell line
Ensembl ID	ENSG00000100644
Description	ARNT-interacting protein (HIF1A) is reported to activate the transcription of NT5E gene, which leads to an increased expression of the drug-metabolizing enzyme Ecto-5'-nucleotidase. As a result, the interaction between HIF1A and NT5E can activate the drug-metabolizing process of Ecto-5'-nucleotidase.
ICD-11: 2C25 Lung cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Non-coding RNA regulation
hsa-miR-30a-5p		Lung cancer		Suppression
miRBase ID	MIMAT0000087
Interaction Name	hsa-miR-30a-5p--NT5E regulation				[2]
Studied Cell Lines	A549 and H226 cell lines
Description	hsa-miR-30a-5p is reported to suppress NT5E mRNA translation by binding to the 3' untranslated region (3'UTR) of NT5E mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Ecto-5'-nucleotidase.
ICD-11: 2C60 Breast cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Histone modification
Histone deacetylases (HDACs)		Breast cancer		Repression
Uniprot ID	HDAC1_HUMAN
Interaction Name	HDACs-NT5E interaction				[3]
Studied Cell Lines	Human breast cancer cell lines
Description	Histone deacetylases (HDACs) are reported to deacetylate the NT5E gene and thereby repress the transcriptional activity of the drug-metabolizing enzyme Ecto-5'-nucleotidase. As a result, the interaction between HDACs and NT5E can inhibit the drug-metabolizing process of Ecto-5'-nucleotidase.

References
1	Ecto-5'-nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia. J Clin Invest. 2002 Oct;110(7):993-1002.
2	CD73/NT5E is a target of miR-30a-5p and plays an important role in the pathogenesis of non-small cell lung cancer. Mol Cancer. 2017 Feb 3;16(1):34.
3	Identification of genes associated with chemosensitivity to SAHA/taxane combination treatment in taxane-resistant breast cancer cells. Breast Cancer Res Treat. 2011 Jan;125(1):55-63.

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