Details of Host Protein-DME Interaction (HOSPPI)

General Information of Drug-Metabolizing Enzyme (DME ID: DME0461)
DME Name	Prolyl 4-hydroxylase alpha-1 (P4HA1), Homo sapiens	DME Info
UniProt ID	P4HA1_HUMAN
EC Number	EC: 1.14.11.2 (Click to Show/Hide the Complete EC Tree) Oxidoreductase Oxygen paired donor oxidoreductase 2-oxoglutarate donor oxidoreductase EC: 1.14.11.2
Lineage	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Interactome

Disease Specific Interactions between Host Protein and DME (HOSPPI)
ICD Disease Classification Healthy
ICD-11: Healthy		Click to Show/Hide the Full List of HOSPPI: 2 HOSPPI
Transcription-factor regulation
Transcription factor Sp1 (SP1)		Health		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-P4HA1 interaction				[2]
Studied Cell Lines	Human aortic smooth muscle cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of P4HA1 gene, which leads to an increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between SP1 and P4HA1 can activate the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
Non-coding RNA regulation
hsa-miR-122-5p		Health		Suppression
miRBase ID	MIMAT0000421
Interaction Name	hsa-miR-122-5p--P4HA1 regulation				[1]
Studied Cell Lines	Hepatic stellate cells
Description	hsa-miR-122-5p is reported to suppress P4HA1 mRNA translation by binding to the 3' untranslated region (3'UTR) of P4HA1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1.
ICD Disease Classification 02 Neoplasms
ICD-11: 2A00 Brain cancer		Click to Show/Hide the Full List of HOSPPI: 4 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Brain neuroblastoma		Significant hypomethylation
Interaction Name	DNMT-P4HA1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 2.48E-10; delta-beta: -3.79E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the P4HA1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between DNMT and P4HA1 can significantly affect the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Brain neuroepithelial tumour		Significant hypomethylation
Interaction Name	DNMT-P4HA1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 2.08E-09; delta-beta: -4.93E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the P4HA1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between DNMT and P4HA1 can significantly affect the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Third ventricle chordoid glioma		Significant hypomethylation
Interaction Name	DNMT-P4HA1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 7.73E-03; delta-beta: -4.89E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the P4HA1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between DNMT and P4HA1 can significantly affect the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Spinal ependymoma		Significant hypomethylation
Interaction Name	DNMT-P4HA1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 5.97E-10; delta-beta: -3.74E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the P4HA1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between DNMT and P4HA1 can significantly affect the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2C12 Liver cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
Non-coding RNA regulation
hsa-miR-30e-5p		Liver cancer		Suppression
miRBase ID	MIMAT0000692
Interaction Name	hsa-miR-30e-5p--P4HA1 regulation				[3]
Studied Cell Lines	HepG2 cell line
Description	hsa-miR-30e-5p is reported to suppress P4HA1 mRNA translation by binding to the 3' untranslated region (3'UTR) of P4HA1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1.
ICD-11: 2C77 Cervical cancer		Click to Show/Hide the Full List of HOSPPI: 2 HOSPPI
Transcription-factor regulation
Upstream stimulatory 1 (USF1)		Cervical cancer		Activation
Uniprot ID	USF1_HUMAN
Interaction Name	USF1-P4HA1 interaction				[4]
Studied Cell Lines	HeLa and Human aortic vascular smooth muscle cell lines
Ensembl ID	ENSG00000158773
Description	Upstream stimulatory 1 (USF1) is reported to activate the transcription of P4HA1 gene, which leads to an increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between USF1 and P4HA1 can activate the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
Upstream stimulatory 2 (USF2)		Cervical cancer		Activation
Uniprot ID	USF2_HUMAN
Interaction Name	USF2-P4HA1 interaction				[4]
Studied Cell Lines	HeLa and Human aortic vascular smooth muscle cell lines
Ensembl ID	ENSG00000105698
Description	Upstream stimulatory 2 (USF2) is reported to activate the transcription of P4HA1 gene, which leads to an increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between USF2 and P4HA1 can activate the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
ICD-11: 2D50 Brain cancer metastasis		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Posterior fossa ependymoma		Significant hypomethylation
Interaction Name	DNMT-P4HA1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 1.10E-94; delta-beta: -6.76E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the P4HA1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Prolyl 4-hydroxylase alpha-1. As a result, the interaction between DNMT and P4HA1 can significantly affect the drug-metabolizing process of Prolyl 4-hydroxylase alpha-1.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual

References
1	miR-122 regulates collagen production via targeting hepatic stellate cells and suppressing P4HA1 expression. J Hepatol. 2013 Mar;58(3):522-8.
2	Adiponectin upregulates prolyl-4-hydroxylase alpha1 expression in interleukin 6-stimulated human aortic smooth muscle cells by regulating ERK 1/2 and Sp1. PLoS One. 2011;6(7):e22819.
3	MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA. Biochem Biophys Res Commun. 2016 Apr 8;472(3):516-22.
4	Human prolyl-4-hydroxylase alpha(I) Transcription Is Mediated by Upstream Stimulatory Factors J Biol Chem. 2006 Apr 21;281(16):10849-55.

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