Details of Host Protein-DME Interaction (HOSPPI)

General Information of Drug-Metabolizing Enzyme (DME ID: DME0496)
DME Name	Phosphoenolpyruvate carboxykinase (PCK1), Homo sapiens	DME Info
UniProt ID	PCKGC_HUMAN
EC Number	EC: 4.1.1.32 (Click to Show/Hide the Complete EC Tree) Lyases Carbon-carbon lyase Carboxy-lyase EC: 4.1.1.32
Lineage	Species: Homo sapiens (Click to Show/Hide the Complete Species Lineage) Kingdom: Metazoa Phylum: Chordata Class: Mammalia Order: Primates Family: Hominidae Genus: Homo Species: Homo sapiens
Interactome

Disease Specific Interactions between Host Protein and DME (HOSPPI)
ICD Disease Classification 02 Neoplasms
ICD-11: 2A00 Brain cancer		Click to Show/Hide the Full List of HOSPPI: 11 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Medulloblastoma		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 7.47E-68; delta-beta: -6.00E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Brain neuroblastoma		Moderate hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypomethylation p-value: 3.73E-09; delta-beta: -2.76E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hypo-methylate the PCK1 gene, which leads to a moderatly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Mixed neuronal-glial tumour		Moderate hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypermethylation p-value: 5.07E-16; delta-beta: 2.67E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hyper-methylate the PCK1 gene, which leads to a moderatly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Cerebral hemispheric glioma		Moderate hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypomethylation p-value: 3.86E-02; delta-beta: -2.17E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hypo-methylate the PCK1 gene, which leads to a moderatly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Melanocytoma		Moderate hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypomethylation p-value: 1.26E-06; delta-beta: -2.68E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hypo-methylate the PCK1 gene, which leads to a moderatly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Cerebellar liponeurocytoma		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 1.35E-04; delta-beta: -3.28E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Diffuse midline glioma		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 1.49E-15; delta-beta: -3.06E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Spinal ependymoma		Significant hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 9.29E-11; delta-beta: 3.14E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the PCK1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Oligodendroglioma		Significant hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 1.35E-24; delta-beta: 3.21E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the PCK1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Atypical teratoid/rhabdoid tumour		Significant hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypermethylation p-value: 5.30E-13; delta-beta: 3.32E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hyper-methylate the PCK1 gene, which leads to a significantly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
DNA methyltransferase (DNMT)		Anaplastic pleomorphic xanthoastrocytoma		Moderate hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypermethylation p-value: 7.41E-04; delta-beta: 2.61E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hyper-methylate the PCK1 gene, which leads to a moderatly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2A01 Meningeal tumour		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Meningioma		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 1.43E-59; delta-beta: -4.12E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD-11: 2C12 Liver cancer		Click to Show/Hide the Full List of HOSPPI: 7 HOSPPI
Transcription-factor regulation
Activating TF factor 2 (ATF2)		Liver cancer		Activation
Uniprot ID	ATF2_HUMAN
Interaction Name	ATF2-PCK1 interaction				[1]
Studied Cell Lines	HepG2 cell line
Ensembl ID	ENSG00000115966
Description	Activating TF factor 2 (ATF2) is reported to activate the transcription of PCK1 gene, which leads to an increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between ATF2 and PCK1 can activate the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
Sterol element-binding 1 (SREBF1)		Liver cancer		Repression
Uniprot ID	SRBP1_HUMAN
Interaction Name	SREBF1-PCK1 interaction				[6]
Studied Cell Lines	HepG2 cell line
Ensembl ID	ENSG00000072310
Description	Sterol element-binding 1 (SREBF1) is reported to repress the transcription of PCK1 gene, which leads to a decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between SREBF1 and PCK1 can repress the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
Transcription factor Sp1 (SP1)		Liver cancer		Activation
Uniprot ID	SP1_HUMAN
Interaction Name	SP1-PCK1 interaction				[6]
Studied Cell Lines	HepG2 cell line
Ensembl ID	ENSG00000185591
Description	Transcription factor Sp1 (SP1) is reported to activate the transcription of PCK1 gene, which leads to an increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between SP1 and PCK1 can activate the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
Non-coding RNA regulation
hsa-miR-26a-1-3p		Liver cancer		Suppression
miRBase ID	MIMAT0004499
Interaction Name	hsa-miR-26a-1-3p--PCK1 regulation				[4]
Studied Cell Lines	Huh7 cell line
Description	hsa-miR-26a-1-3p is reported to suppress PCK1 mRNA translation by binding to the 3' untranslated region (3'UTR) of PCK1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase.
hsa-miR-33b-5p		Liver cancer		Suppression
miRBase ID	MIMAT0003301
Interaction Name	hsa-miR-33b-5p--PCK1 regulation				[5]
Studied Cell Lines	HepG2 cell line
Description	hsa-miR-33b-5p is reported to suppress PCK1 mRNA translation by binding to the 3' untranslated region (3'UTR) of PCK1 mRNA, which leads to a decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase.
Histone modification
Histone acetyltransferases (HATs)		Liver cancer		Activation
Uniprot ID	HAT1_HUMAN
Interaction Name	HATs-PCK1 interaction				[2]
Studied Cell Lines	HepG2 cell line
Description	Histone acetyltransferases (HATs) are reported to acetylate the PCK1 gene and thereby activate the transcriptional activity of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between HATs and PCK1 can enhance the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
Histone deacetylase 4 (HDAC4)		Liver cancer		Repression
Uniprot ID	HDAC4_HUMAN
Interaction Name	HDAC4-PCK1 interaction				[3]
Studied Cell Lines	HepG2 cell line
Description	Histone deacetylase 4 (HDAC4) is reported to deacetylate the PCK1 gene and thereby represses the transcriptional activity of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between HDAC4 and PCK1 can inhibit the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
ICD-11: 2C25 Lung cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Lung cancer		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 8.79E-21; delta-beta: -3.04E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
The Methylation Level of Disease Section Compare with the Other Disease Section	Significant hypomethylation p-value: 1.80E-07; delta-beta: -3.70E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals DME methylation in tissue other than the diseased tissue of patients
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual Download DME methylation data in the tissue of the other disease section
ICD-11: 2F37 Endocrine gland cancer		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Pituitary adenoma		Moderate hypermethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Moderate hypermethylation p-value: 1.45E-07; delta-beta: 2.64E-01
Description	DNA methyltransferase (DNMT) is reported to moderatly hyper-methylate the PCK1 gene, which leads to a moderatly decreased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can moderatly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual
ICD Disease Classification 05 Endocrine/nutritional/metabolic diseases
ICD-11: 5B81 Obesity		Click to Show/Hide the Full List of HOSPPI: 1 HOSPPI
DNA methylation
DNA methyltransferase (DNMT)		Obesity		Significant hypomethylation
Interaction Name	DNMT-PCK1 interaction
The Methylation Level of Disease Section Compare with the Healthy Individual Tissue	Significant hypomethylation p-value: 1.74E-15; delta-beta: -4.15E-01
Description	DNA methyltransferase (DNMT) is reported to significantly hypo-methylate the PCK1 gene, which leads to a significantly increased expression of the drug-metabolizing enzyme Phosphoenolpyruvate carboxykinase. As a result, the interaction between DNMT and PCK1 can significantly affect the drug-metabolizing process of Phosphoenolpyruvate carboxykinase.
DME methylation in the diseased tissue of patients DME methylation in the normal tissue of healthy individuals
Violin Diagram of DME Disease-specific Methylation Level			Click to View the Clearer Original Diagram
Download DME methylation barchart for all samples Download DME methylation data of patients in the disease section of the tissue Download DME methylation data in the tissue of healthy individual

References
1	Activating transcription factor-2 mediates transcriptional regulation of gluconeogenic gene PEPCK by retinoic acid. Diabetes. 2002 Dec;51(12):3400-7.
2	Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis. Cell. 2009 Mar 20;136(6):1073-84.
3	Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4. Diabetes Metab Syndr Obes. 2019 Mar 25;12:401-411.
4	MicroRNA-26a regulates insulin sensitivity and metabolism of glucose and lipids. J Clin Invest. 2015 Jun;125(6):2497-509.
5	MicroRNA 33 regulates glucose metabolism. Mol Cell Biol. 2013 Aug;33(15):2891-902.
6	SREBP-1c and Sp1 interact to regulate transcription of the gene for phosphoenolpyruvate carboxykinase (GTP) in the liver. J Biol Chem. 2004 Apr 9;279(15):15385-95.

If you find any error in data or bug in web service, please kindly report it to Dr. Yin and Dr. Li.