Details of Drug-Metabolizing Enzyme (DME)

General Information of DME (ID: DMEN039)
DME Name Bifunctional trans-3-hydroxy-L-proline dehydratase/2-epimerase (lhpL), Pseudomonas aeruginosa
Gene Name lhpL
UniProt ID
T3HPD_PSEAE
EC Number    EC: 2.7.1.35     (Click to Show/Hide the Complete EC Tree)
Transferase
Kinase
Phosphotransferase
EC: 2.7.1.35
Lineage    Species: Pseudomonas aeruginosa     (Click to Show/Hide the Complete Species Lineage)
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species: Pseudomonas aeruginosa
      Click to Show/Hide the Molecular/Functional Data (Sequence/Structure/Pathway/Function) of This DME
Sequence
MRSQRIVHIVSCHAEGEVGDVIVGGVAAPPGATLWEQSRWIARDQDLRNFVLNEPRGGVF
RHANLLVPAKDPRAQMGWIIMEPADTPPMSGSNSLCVATVLLDSGILPMREPLTRLLLEA
PGGLIEARAECRDGKAERVEIRNVPSFADRLDAWIEVEGLGSLQVDTAYGGDSFVIADAR
RLGFALRADEAAELVATGLKITHAANEQLGFRHPTNPDWDHLSFCQLAAPPERRDGVLGA
NNAVVIRPGKIDRSPCGTGCSARMAVLQAKGQLRVGERFVGRSIIGSEFHCHIESLTELG
GRPAILPCLSGRAWITGIHQYLLDPDDPWPQGYRLSDTWPGGHC
Function Bifunctional enzyme catalyzing both the dehydration of trans-3-hydroxy-L-proline (t3LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C) and 2-epimerization of t3LHyp to cis-3-hydroxy-D-proline (c3DHyp). No dehydratase activity with L-proline, trans-4-hydroxy-L-proline (t4LHyp), cis-4-hydroxy-L-proline (c4LHyp), D-proline, cis-4-hydroxy-D-proline (c4DHyp), trans-4-hydroxy-D-proline (t4DHyp) or L-serine as substrates. Displays neither t4LHyp epimerase nor proline racemase activity. Is likely involved in a degradation pathway that converts t3LHyp to L-proline, which would allow P.aeruginosa to grow on t3LHyp as a sole carbon source.
Full List of Drug(s) Metabolized by This DME
      Preclinical/investigative Agents Click to Show/Hide the Full List of Drugs:          1 Drugs
AK-41099
 Drug Info Investigative Discovery agent ICD: N.A. [1]
Full List of Metabolic Reactions (MR) Catalyzed by This DME
MR ID Reactant Product MR Type Source Drug REF
MR003201 Delta2-Pyrroline-2-carboxylate Pyr2C Unclear - Unclear AK-41099 [2]
MR003202 Pyr2C L-proline Unclear - Unclear AK-41099 [2]
References
1 Structure of Thermococcus litoralis trans-3-hydroxy-l-proline dehydratase in the free and substrate-complexed form Biochem Biophys Res Commun. 2019 Aug 13;516(1):189-195. doi: 10.1016/j.bbrc.2019.06.021.
2 Structure of Thermococcus litoralis trans-3-hydroxy-l-proline dehydratase in the free and substrate-complexed form. Biochem Biophys Res Commun. 2019 Aug 13;516(1):189-195.

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