Details of Drug-Metabolizing Enzyme (DME)

General Information of DME (ID: DME1201)
DME Name Alpha-L-rhamnosidase (lrA), Bifidobacterium dentium
Synonyms Alpha-L-rhamnosidase T; Alpha-L-rhamnosidase N; Alpha-L-rhamnoside rhamnohydrolase; B5790_0114
Gene Name lrA
UniProt ID
A0A1V8Q7P3_9BIF
EC Number    EC: 3.2.1.40     (Click to Show/Hide the Complete EC Tree)
Hydrolases
Glycosylase
O/S-glycosyl compound glycosidase
EC: 3.2.1.40
Lineage    Species: Bifidobacterium dentium     (Click to Show/Hide the Complete Species Lineage)
Kingdom: Bacteria
Phylum: Actinobacteria
Class: Actinobacteria
Order: Bifidobacteriales
Family: Bifidobacteriaceae
Genus: Bifidobacterium
Species: Bifidobacterium dentium
      Click to Show/Hide the Molecular/Functional Data (Sequence/Structure/Pathway/Function) of This DME
Tissue Distribution Primarily distributed in human gut.
Sequence
MDTQIIKPIAVGDLHITRFTIEHFPGDALGIPTATPRMNWTYSRTVPDGSQILLKISRRI
PGAQAREEYTYLPPEDNVLVPWQFTPLASREEVFATVQLVSAAHKPLSDPSATLHFEAGL
MQEYEHVADFVGPSWAEPETDHRHLPLVRTEIAIKAKPKRARLYLSALGLVEAEINGVKI
GNDALIPGWTNYEQRVQCWTYDVTDELNEGANAMGFWLGDGWYRGRLGFDGGYVNYYGDR
LGVFAQLEIEYEDGGIEHVYSNAWDRQWKASLGPIVCSDLCEGERYDARLEQPGWSEPGF
DDSNWKPVSEIFYDPAKIENPDTSPVRAHEINEPVSIARTGDTPDGRGIWLVDFGQNCTQ
RIRLRIAGLEAGQSVELHHVEVLEPNGEIATRTLRRGQQHDIYTSNGTDAWWEPRFAMHG
FRYAQIEGVVGELTVADIECHVYHSVMDRAGELETSNPLLNRLHANALWSMRSNFVSVPQ
DCPQRDERMGWTGDICLFAPTAAYLYDVYGFLKSWLKDVRADQVKWGTVPFYVPFIPLGG
WAHPQAISTWGDSAVEVPWVLYMESGDPQILADSYELSRDWIDEVAGYLSADGVWDRRPD
YVLGQLGDWLDPTAPPDDPTQAMTEKELVATAFFARSCAQAEQIAQILGKSEDVVRFGDL
RKHVTSGFLGRFTNLDGTMTSDTQCAYALAIAFGLLDGEPVRRVKAGNRLAKLVRESGGK
VSTGFAGTPFVLPALTMTGHNEEAYGLLLSEECPSWLYQVKMGATTTWERWDSMRPDGSL
NPGGMTSFNHYALGSVAEWMHAHIGGLEAIEAGWKRFRVAPVIGGGIDHASTSHVTPYGK
ASVAWKTSETGVELDVTVPIGATAVVELPDHEPTELIGGTHHLVVAL
Function This enzyme utilizes an inverting mechanism of hydrolysis, releasing beta-L-rhamnose. Substrates include naringin, rutin, quercitrin, hesperidin, dioscin, terpenyl glycosides and many other natural glycosides containing terminal alpha-L-rhamnose.
Full List of Drug(s) Metabolized by This DME
      Drugs in Phase 2 Clinical Trial Click to Show/Hide the Full List of Drugs:          1 Drugs
BRN-0075455
 Drug Info Phase 2/3 Diabetes mellitus ICD11: 5A10 [1]
      Preclinical/investigative Agents Click to Show/Hide the Full List of Drugs:          4 Drugs
HY-N2258
 Drug Info Preclinical Anaesthesia ICD11: 8E22 [1]
Naringin
 Drug Info Investigative Ovarian cancer ICD11: 2C73 [1]
Ginsenoside Re
 Drug Info Investigative Lung cancer ICD11: 2C25 [1]
PNA-l-rhamnopyranoside
 Drug Info Investigative Discovery agent ICD: N.A. [1]
      Experimental Enzyme Kinetic Data of Drugs Click to Show/Hide the Full List of Drugs:          5 Drugs
BRN-0075455
 Drug Info Phase 2/3 Diabetes mellitus Km = 2190 microM [1]
HY-N2258
 Drug Info Preclinical Anaesthesia Km = 370 microM [1]
Naringin
 Drug Info Investigative Ovarian cancer Km = 320 microM [1]
Ginsenoside Re
 Drug Info Investigative Lung cancer Km = 860 microM [1]
PNA-l-rhamnopyranoside
 Drug Info Investigative Discovery agent Km = 1060 microM [1]
Full List of Metabolic Reactions (MR) Catalyzed by This DME
MR ID Reactant Product MR Type Source Drug REF
MR006511 BRN-0075455 AS-35335 Oxidation - Deglycosylation BRN-0075455 [2]
References
1 Metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing alpha-L-rhamnosidase from Bifidobacterium dentium. J Microbiol Biotechnol. 2015 Jan;25(1):18-25.
2 Identification of rutin deglycosylated metabolites produced by human intestinal bacteria using UPLC-Q-TOF/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jun 1;898:95-100.

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