Details of Drug-Metabolizing Enzyme (DME)

General Information of DME (ID: DMEN356)
DME Name Steroid C26-monooxygenase (cyp142A1), Mycobacterium tuberculosis
Gene Name cyp142A1
UniProt ID
CP142_MYCTU
EC Number    EC: 1.14.15.28     (Click to Show/Hide the Complete EC Tree)
Oxidoreductase
Oxygen paired donor oxidoreductase
Iron-sulfur protein donor oxidoreductase
EC: 1.14.15.28
Lineage    Species: Mycobacterium tuberculosis     (Click to Show/Hide the Complete Species Lineage)
Kingdom: .
Phylum: .
Class: .
Order: .
Family: .
Genus: .
Species: Mycobacterium tuberculosis
      Click to Show/Hide the Molecular/Functional Data (Sequence/Structure/Pathway/Function) of This DME
Sequence
MTEAPDVDLADGNFYASREARAAYRWMRANQPVFRDRNGLAAASTYQAVIDAERQPELFS
NAGGIRPDQPALPMMIDMDDPAHLLRRKLVNAGFTRKRVKDKEASIAALCDTLIDAVCER
GECDFVRDLAAPLPMAVIGDMLGVRPEQRDMFLRWSDDLVTFLSSHVSQEDFQITMDAFA
AYNDFTRATIAARRADPTDDLVSVLVSSEVDGERLSDDELVMETLLILIGGDETTRHTLS
GGTEQLLRNRDQWDLLQRDPSLLPGAIEEMLRWTAPVKNMCRVLTADTEFHGTALCAGEK
MMLLFESANFDEAVFCEPEKFDVQRNPNSHLAFGFGTHFCLGNQLARLELSLMTERVLRR
LPDLRLVADDSVLPLRPANFVSGLESMPVVFTPSPPLG
Structure
2XKR ; 7P5T ; 7QQ7
Function Involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain during infection . Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4-en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate . In vitro, Cyp142 catalyzes with equal preference the oxidation of both (25R)- and (25S)-26-hydroxycholest-4-en-3-one diastereomers to the corresponding carboxylic acid which is a prerequisite for entry into the beta-oxidation pathway . Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one .
Full List of Drug(s) Metabolized by This DME
      Drugs in Phase 2 Clinical Trial Click to Show/Hide the Full List of Drugs:          1 Drugs
ANW-32821
 Drug Info Phase 2 Cone rod dystrophy ICD11: 9B70 [1], [2]
Full List of Metabolic Reactions (MR) Catalyzed by This DME
MR ID Reactant Product MR Type Source Drug REF
MR007245 27-hydroxycholesterol Carboxylic acid Unclear - Unclear ANW-32821 [1], [2]
MR007244 ANW-32821 27-hydroxycholesterol Unclear - Unclear ANW-32821 [1], [2]
References
1 Cholesterol ester oxidation by mycobacterial cytochrome P450. J Biol Chem. 2014 Oct 31;289(44):30417-25.
2 Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets? Appl Microbiol Biotechnol. 2019 May;103(9):3597-3614.

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